Human Vitamin D Receptor, VD R ELISA Kit | MyBioSource MBS704497 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Vitamin D Receptor, VD R ELISA Kit

catalog :

MBS704497

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704497

products type :

ELISA Kit

products full name :

Human Vitamin D Receptor, VD R ELISA Kit

products short name :

vitamin D (1, 25- dihydroxyvitamin D3) receptor

products name syn :

Human Vitamin D Receptor; VD R ELISA Kit; NR1I1; vitamin D (1; 25-dihydroxyvitamin D3) receptor; vitamin D (1; 25- dihydroxyvitamin D3) receptor

other names :

vitamin D3 receptor isoform VDRA; Vitamin D3 receptor; vitamin D3 receptor; 1,25-dihydroxyvitamin D3 receptor; vitamin D nuclear receptor variant 1; nuclear receptor subfamily 1 group I member 1; protein phosphatase 1, regulatory subunit 163; vitamin D (1,25- dihydroxyvitamin D3) receptor; 1,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1

products gene name :

VDR

other gene names :

VDR; VDR; NR1I1; PPP1R163; NR1I1; VDR

uniprot entry name :

VDR_HUMAN

reactivity :

Human

sequence length :

427

specificity :

This assay has high sensitivity and excellent specificity for detection of human VD R. No significant cross-reactivity or interference between human VD R and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Detection Range: 6.25 pg/ml -400 pg/ml. Sensitivity: The minimum detectable dose of human VD R is typically less than 1.56 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VD R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VD R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VD R is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VD R bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4507883

ncbi acc num :

NP_000367.1

ncbi gb acc num :

NM_000376.2

uniprot acc num :

P11473

ncbi mol weight :

48,289 Da

ncbi pathways :

Direct P53 Effectors Pathway (137939); Drug Induction Of Bile Acid Pathway (698755); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213307); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Nuclear Receptor Transcription Pathway (105979); Nuclear Receptors Pathway (198848)

ncbi summary :

This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Feb 2011]

uniprot summary :

Function: Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.4 PublicationsManual assertion based on experiment in:Ref.15

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage