catalog number :
MBS704489
products type :
ELISA Kit
products full name :
Rat microtubule-associated protein 2, MAP-2 ELISA Kit
products short name :
microtubule-associated protein 2
products name syn :
Rat microtubule-associated protein 2; MAP-2 ELISA Kit; DKFZp686I2148; MAP2A; MAP2B; MAP2C; OTTHUMP00000163916; microtubule-associated protein 2
other names :
Microtubule-associated protein 2; microtubule-associated protein 2; microtubule-associated protein 2
products gene name :
MAP2
other gene names :
Map2; Map2; MAP2R; Mtap2; Mtap2; MAP-2
uniprot entry name :
MTAP2_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat MAP-2. No significant cross-reactivity or interference between rat MAP-2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of rat MAP-2 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MAP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
XP_006245164.1
ncbi gb acc num :
XM_006245102.1
ncbi mol weight :
202,411 Da
ncbi pathways :
MAPK Cascade Pathway (198535)
ncbi summary :
regulates and organizes microtubules; may serve as a scaffolding protein for neuromodulatory activities [RGD, Feb 2006]
uniprot summary :
Function: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
size4 :
10x96-Strip-Wells