ELISA/assay

Bovine Immunoglobulin G, IgG ELISA Kit

MBS704485

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Bovine Immunoglobulin G, IgG ELISA Kit

[Immunoglobulin G, IgG]

[Bovine Immunoglobulin G; IgG ELISA Kit; Bovine Immunoglobulin G; IgG]

[IgG]

[IgG]

Bovine

476

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernates and tissue homogenates. Detection Range: 9.4 ug/ml-600 ug/ml. Sensitivity: 7.397 ug/ml

Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunoglobulin

Introduction: Immunoglobulin G (IgG) is a multimeric immunoglobulin, built of two heavy chains gamma and two light chains. Each complex has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. IgG molecules are synthesised and secreted by plasma B cells. IgG antibodies are predominately involved in the secondary antibody response, (the main antibody involved in primary response is IgM) which occurs approximately one month following antigen recognition, thus the presence of specific IgG generally corresponds to maturation of the antibody response. Pro-inflammatory cytokines particularly IL-4 and IL-2, have a crucial role in activation of the IgG antibody response.This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in utero. Along with IgA secreted in the breast milk, residual IgG absorbed through the placenta provides the neonate with humoral immunity before its own immune system develops.It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by agglutination and immobilization, complement activation (classical pathway), opsonization for phagocytosis and neutralization of their toxins. It also plays an important role in Antibody-dependent cell-mediated cytotoxicity(ADCC). IgG is also associated with Type II and Type III Hypersensitivity. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with bovine IgG. Standards or samples are then added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) -conjugated antibody preparation specific for bovine IgG, mix well and incubated. The more the amount of bovine IgG in samples, the less HRP- conjugated antibody preparation specific for bovine IgG bound by pre-coated bovine IgG. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. And the color develops in opposite to the amount of bovine IgG in the sample. The color development is stopped and the intensity of the color is measured.

185362

AAA02914.1

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24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

310

96-Strip-Wells

425

5x96-Strip-Wells

1795

10x96-Strip-Wells

3380