PPM1D Antibody | MyBioSource MBS7044849 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PPM1D Antibody

catalog :

MBS7044849

quantity :

0.05 mL

price :

120 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot. All lanes: PPM1Dantibody at 2.66ug/ml. Lane 1 : Rat gonadal tissue. Lane 2 : U87 whole cell lysate. Lane 3 : 293T whole cell lysate. Secondary. Goat polyclonal to Rabbit IgG at 1/10000 dilution. Predicted band size: 67,48 kDa. Observed band size: 67 kDa.

image 2 :

Immunohistochemistry of paraffin-embedded human liver cancer using MBS7044849 at dilution 1:100

image 3 :

Immunohistochemistry of paraffin-embedded human small intestine using MBS7044849 at dilution 1:100

product information

catalog number :

MBS7044849

products type :

Antibody

products full name :

PPM1D Antibody

products short name :

[PPM1D]

products name syn :

[Protein phosphatase 1D; Protein phosphatase 2C isoform delta; PP2C-delta; Protein phosphatase magnesium-dependent 1 delta; p53-induced protein phosphatase 1; PPM1D; WIP1]

other names :

[protein phosphatase 1D; Protein phosphatase 1D; protein phosphatase 1D; protein phosphatase, Mg2+/Mn2+ dependent 1D; Protein phosphatase 2C isoform delta; PP2C-delta; Protein phosphatase magnesium-dependent 1 delta; p53-induced protein phosphatase 1]

products gene name :

[PPM1D]

other gene names :

[PPM1D; PPM1D; WIP1; PP2C-DELTA; WIP1; PP2C-delta]

uniprot entry name :

PPM1D_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Rat

sequence length :

605

purity :

Antigen Affinity Purified

form :

Liquid

storage stability :

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Recommendeddilution:. WB: 1:1000-1:5000. IHC: 1:20-1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Species: Human. Immunogen: Recombinant human Protein phosphatase 1D protein (N-250AA)

other info2 :

Conjugate: Non-conjugated. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-69130 / sc-69131 / sc-20712

products description :

Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation (PubMed:21283629).

ncbi gi num :

4505997

ncbi acc num :

NP_003611.1

ncbi gb acc num :

NM_003620.3

uniprot acc num :

O15297

ncbi pathways :

P53 Pathway (138067); P53 Signaling Pathway (83055); P53 Signaling Pathway (465)

ncbi summary :

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq, Jul 2008]

uniprot summary :

PPM1D: a magnesium-dependent Ser/Thr phosphatase. A p53-induced phosphatase that interacts with the nuclear isoform of uracil DNA glycosylase, UNG2, and suppresses base excision repair. Might contribute to growth inhibitory pathways activated in response to DNA damage in a p53-dependent manner. Protein type: Nuclear receptor co-regulator; Cell cycle regulation; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 17q23.2. Cellular Component: nucleus. Molecular Function: mitogen-activated protein kinase binding; protein binding; protein serine/threonine phosphatase activity. Biological Process: negative regulation of cell proliferation; protein amino acid dephosphorylation; response to radiation. Disease: Breast Cancer

size1 :

0.05 mL

price1 :

120 USD

size2 :

0.1 mL

price2 :

160

more info or order :

MyBioSource product webpage