ELISA/assay

Canine bone morphogenetic protein 2, BMP-2 ELISA Kit

MBS704468

96 Strip Wells

915 USD

ELISA Kit

Canine bone morphogenetic protein 2, BMP-2 ELISA Kit

bone morphogenetic protein 2, BMP-2

Canine bone morphogenetic protein 2; BMP-2 ELISA Kit; bone morphogenetic protein 2; BMP-2

bone morphogenetic protein 2 preproprotein; Bone morphogenetic protein 2; bone morphogenetic protein 2; BMP-2A; bone morphogenetic protein 2A; bone morphogenetic protein 2; Bone morphogenetic protein 2A; BMP-2A

BMP-2

BMP2; BMP2; BDA2; BMP2A; BMP2A; BMP-2; BMP-2A

BMP2_HUMAN

Dog

396

This assay has high sensitivity and excellent specificity for detection of canine BMP-2. No significant cross-reactivity or interference between canine BMP-2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of canine bone morphogenetic protein 2 (BMP-2) concentrations in serum, plasma, tissue homogenates. Detection Range: 62.5 pg/ml-4000 pg/ml.

Sensitivity: The minimum detectable dose of canine BMP-2 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: </b<This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BMP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4557369

NP_001191.1

NM_001200.2

P12643

44,702 Da

Adipogenesis Pathway 198832!!BDNF Signaling Pathway 712093!!BMP Signalling And Regulation Pathway 198910!!Basal Cell Carcinoma Pathway 83113!!Basal Cell Carcinoma Pathway 525!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Elastic Fibre Formation Pathway 730310!!Extracellular Matrix Organization Pathway 576262!!Heart Development Pathway 198802

The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008]

Function: Induces cartilage and bone formation.

96 Strip Wells

915 USD