catalog number :
MBS704467
products type :
ELISA Kit
products full name :
Bovine Lactoferrin, LTF/LF ELISA Kit
products short name :
[lactotransferrin]
products name syn :
[Bovine Lactoferrin; LTF/LF ELISA kit; GIG12; HLF2; LF; growth-inhibiting protein 12; lactoferrin; neutrophil lactoferrin; talalactoferrin; lactotransferrin]
other names :
[lactotransferrin; Lactotransferrin; lactotransferrin; lactoferrin; lactotransferrin]
products gene name :
[LTF]
other gene names :
[LTF; LTF; Lf; Lactoferrin; Lfcin-B]
uniprot entry name :
TRFL_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of sheep LF/LTF. No significant cross-reactivity or interference between sheep LF/LTF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Breast Milk. Assay Type: Competitive. Detection Range: 0.156 ug/ml-10 ug/ml. Sensitivity: 0.078 ug/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LF/LTF. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated LF/LTF. The competitive inhibition reaction is launched between with HRP-conjugated LF/LTF and LF/LTF in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of LF/LTF in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_851341.1
ncbi gb acc num :
NM_180998.2
ncbi mol weight :
78,056 Da
ncbi pathways :
Amyloids Pathway (1008671); Disease Pathway (942846); Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway (1008667); Phagosomal Maturation (early Endosomal Stage) Pathway (1008668)
uniprot summary :
Function: Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate.5 PublicationsManual assertion based on experiment in:Ref.8. Catalytic activity: Preferential at -Arg-Ser-Arg-Arg- - and -Arg-Arg-Ser-Arg- -, and of Z-Phe-Arg- -aminomethylcoumarin. Enzyme regulation: Inhibited by PMSF and Pefabloc.
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells