Human urokinase plasminogen activator, uPA ELISA Kit | MyBioSource MBS704460 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human urokinase plasminogen activator, uPA ELISA Kit

catalog :

MBS704460

quantity :

96-Strip-Wells

price :

680 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704460

products type :

ELISA Kit

products full name :

Human urokinase plasminogen activator, uPA ELISA Kit

products short name :

urokinase plasminogen activator, uPA

products name syn :

Human urokinase plasminogen activator; uPA ELISA kit; Human urokinase plasminogen activator; uPA

other names :

urokinase plasminogen activator, partial; Urokinase-type plasminogen activator; urokinase-type plasminogen activator; U-plasminogen activator; plasminogen activator, urinary; plasminogen activator, urokinase

products gene name :

uPA

other gene names :

PLAU; PLAU; ATF; QPD; UPA; URK; u-PA; BDPLT5; U-plasminogen activator; uPA

uniprot entry name :

UROK_HUMAN

reactivity :

Human

sequence length :

411

specificity :

This assay has high sensitivity and excellent specificity for detection of human uPA. No significant cross-reactivity or interference between human uPA and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 78 pg/ml-5000 pg/ml. Sensitivity: The minimum detectable dose of human uPA is typically less than 19.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for uPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any uPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for uPA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of uPA bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

56068043

ncbi acc num :

AAV70488.1

uniprot acc num :

P00749

ncbi mol weight :

46,908 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); DNA Damage Response (only ATM Dependent) Pathway (198827); Dissolution Of Fibrin Clot Pathway (106061); E2F Transcription Factor Network Pathway (137934); Endochondral Ossification Pathway (198812); FGF Signaling Pathway (137989)

ncbi summary :

This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

uniprot summary :

Function: Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. Catalytic activity: Specific cleavage of Arg- -Val bond in plasminogen to form plasmin. Enzyme regulation: Inhibited by SERPINA5.2 PublicationsManual assertion based on experiment in:Ref.17

size1 :

96-Strip-Wells

price1 :

680 USD

size2 :

5x96-Strip-Wells

price2 :

2140

size3 :

10x96-Strip-Wells

price3 :

3990

more info or order :

MyBioSource product webpage