product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
human Endothelin 1, ET-1 ELISA Kit
catalog :
MBS704440
quantity :
96 Strip Wells
price :
760 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS704440
products type :
ELISA Kit
products full name :
human Endothelin 1, ET-1 ELISA Kit
products short name :
endothelin 1
products name syn :
human Endothelin 1; ET-1 ELISA Kit; ET1; HDLCQ7; preproendothelin 1; endothelin 1
other names :
endothelin-1; Endothelin-1; endothelin-1; ET-1; PPET1; preproendothelin-1; endothelin 1<; Preproendothelin-1; PPET1
products gene name :
EDN1
other gene names :
EDN1; EDN1; ET-1; PPET1
uniprot entry name :
EDN1_BOVIN
reactivity :
Human
sequence length :
202
specificity :
This assay has high sensitivity and excellent specificity for detection of Human EDN1. No significant cross-reactivity or interference between Human EDN1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, cell culture supernates, urine, saliva. Detection Range: 3.12 pg/ml-200 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 0.78 pg/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ET-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ET-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ET-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ET-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
30794314
ncbi acc num :
NP_851353.1
ncbi gb acc num :
NM_181010.2
uniprot acc num :
P17322
ncbi mol weight :
22,977 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway 1008554!!G Alpha (q) Signalling Events Pathway 1008586!!GPCR Downstream Signaling Pathway 1008582!!GPCR Ligand Binding Pathway 1008553!!Gastrin-CREB Signalling Pathway Via PKC And MAPK 1008603!!HIF-1 Signaling Pathway 695207!!Melanogenesis Pathway 84331!!Melanogenesis Pathway 504!!Peptide Ligand-binding Receptors Pathway 1008555!!Prostaglandin Synthesis And Regulation Pathway 920923
uniprot summary :
Function: Endothelins are endothelium-derived vasoconstrictor peptides.
size :
96 Strip Wells
price :
760 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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