catalog number :
MBS704435
products type :
ELISA Kit
products full name :
Sheep Interleukin 10, IL-10 ELISA Kit
products short name :
[interleukin 10]
products name syn :
[Sheep Interleukin 10; IL-10 ELISA Kit; CSIF; IL-10; IL10A; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor; interleukin 10]
other names :
[interleukin-10; Interleukin-10; interleukin-10; CSIF; IL-10; cytokine synthesis inhibitory factor; Cytokine synthesis inhibitory factor; CSIF]
products gene name :
[IL10]
other gene names :
[IL10; IL10; IL-10; CSIF]
uniprot entry name :
IL10_SHEEP
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine IL-1. No significant cross-reactivity or interference between bovine IL-1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Tissue Homogenates. Assay Type: Competitive. Detection Range: 250 pg/ml-4000 pg/ml. Sensitivity: 250 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-1. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated IL-1. A competitive inhibition reaction is launched between IL-1 (Standards or samples) and Biotin-conjugated IL-1 with the pre-coated antibody specific for IL-1. The more amount of IL-1 in samples, the less antibody bound by Biotin-conjugated IL-1. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of IL-1 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001009327.1
ncbi gb acc num :
NM_001009327.1
ncbi mol weight :
20,189 Da
uniprot summary :
Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells