DDX11 Antibody | MyBioSource MBS7044341 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

DDX11 Antibody

catalog :

MBS7044341

quantity :

0.05 mL

price :

120 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot. All lanes: DDX11 antibody at 8.47 ug/ml. Lane 1: Mouse gonadal tissue. Lane 2: K562 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 109, 102, 99, 97, 33 kDa. Observed band size: 109 kDa.

image 2 :

Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS7044341 at dilution 1:100

image 3 :

Immunohistochemistry of paraffin-embedded human gastric cancer using MBS7044341 at dilution 1:100

product information

catalog number :

MBS7044341

products type :

Antibody

products full name :

DDX11 Antibody

products short name :

[DDX11]

products name syn :

[ATP-dependent DNA helicase DDX11 (EC 3.6.4.12) (CHL1-related protein 1) (hCHLR1) (DEAD/H-box protein 11) (Keratinocyte growth factor-regulated gene 2 protein) (KRG-2), DDX11, CHL1 CHLR1 KRG2]

other names :

[probable ATP-dependent DNA helicase DDX11 isoform 3; Probable ATP-dependent DNA helicase DDX11; probable ATP-dependent DNA helicase DDX11; DEAD/H-box helicase 11; CHL1-related protein 1; hCHLR1; DEAD/H box protein 11; Keratinocyte growth factor-regulated gene 2 protein; KRG-2]

products gene name :

[DDX11]

other gene names :

[DDX11; DDX11; CHL1; KRG2; WABS; CHLR1; CHL1; CHLR1; KRG2; hCHLR1; KRG-2]

uniprot entry name :

DDX11_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

970

purity :

Antigen Affinity Purified

form :

Liquid

storage stability :

Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Species: Human. Immunogen: Recombinant human Probable ATP-dependent DNA helicase DDX11 protein (N-220AA). Research Area: Cell Biology

other info2 :

Conjugate: Non-conjugated. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-68468 / sc-68855

products description :

DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitment of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA.

ncbi gi num :

380420356

ncbi acc num :

NP_001244073.1

ncbi gb acc num :

NM_001257144.1

uniprot acc num :

Q96FC9

ncbi pathways :

IRE1alpha Activates Chaperones Pathway (1268759); Metabolism Of Proteins Pathway (1268677); Unfolded Protein Response (UPR) Pathway (1268756); XBP1(S) Activates Chaperone Genes Pathway (1268760)

ncbi summary :

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

DDX11: DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitment of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA. Defects in DDX11 are the cause of Warsaw breakage syndrome (WBRS). It is a syndrome characterized by severe microcephaly, pre- and postnatal growth retardation, facial dysmorphism and abnormal skin pigmentation. Additional features include high arched palate, coloboma of the right optic disk, deafness, ventricular septal defect, toes and fingers abnormalities. At cellular level, drug-induced chromosomal breakage, a feature of Fanconi anemia, and sister chromatid cohesion defects, a feature of Roberts syndrome, coexist. Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Nucleolus; Helicase; EC 3.6.4.13. Chromosomal Location of Human Ortholog: 12p11. Cellular Component: midbody; nuclear chromatin; nucleolus; nucleoplasm; nucleus; spindle pole. Molecular Function: DNA-dependent ATPase activity; double-stranded DNA binding; helicase activity; protein binding; single-stranded DNA binding. Biological Process: sister chromatid cohesion. Disease: Warsaw Breakage Syndrome

size1 :

0.05 mL

price1 :

120 USD

size2 :

0.1 mL

price2 :

160

more info or order :

MyBioSource product webpage