Rat hypoxia-inducible factor 1alpha, HIF-1alpha ELISA Kit | MyBioSource MBS704416 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat hypoxia-inducible factor 1alpha, HIF-1alpha ELISA Kit

catalog :

MBS704416

quantity :

48-Strip-Wells

price :

605 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704416

products type :

ELISA Kit

products full name :

Rat hypoxia-inducible factor 1alpha, HIF-1alpha ELISA Kit

products short name :

hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

products name syn :

Rat hypoxia-inducible factor 1alpha; HIF-1alpha ELISA Kit; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78; ARNT interacting protein; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1; alpha subunit; hypoxia-inducible factor 1; alpha; hypoxia inducible factor 1; alpha subunit (basic helix-loop-helix transcription factor)

other names :

hypoxia-inducible factor 1-alpha; Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor 1-alpha; HIF1 alpha; HIF1-alpha; HIF-1 alpha; HIF-1-alpha; hypoxia-inducible factor 1 alpha; hypoxia inducible factor 1, alpha subunit; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

products gene name :

HIF1A

other gene names :

Hif1a; Hif1a; MOP1; HIF-1-alpha; HIF1-alpha

uniprot entry name :

HIF1A_RAT

reactivity :

Rat

sequence length :

823

specificity :

This assay has high sensitivity and excellent specificity for detection of rat HIF-1alpha. No significant cross-reactivity or interference between rat HIF-1alpha and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: The minimum detectable dose of rat HIF-1alpha is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HIF-1alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIF-1alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIF-1alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIF-1alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

13242249

ncbi acc num :

NP_077335.1

ncbi gb acc num :

NM_024359.1

uniprot acc num :

O35800

ncbi mol weight :

92,319 Da

ncbi pathways :

Adipogenesis Pathway (198479); HIF-1 Signaling Pathway (695233); Pathways In Cancer (83493); Proteoglycans In Cancer Pathway (782034); Proteoglycans In Cancer Pathway (782054); Renal Cell Carcinoma Pathway (83495); Renal Cell Carcinoma Pathway (519); Thyroid Hormone Signaling Pathway (946618); MTOR Signaling Pathway (83451); MTOR Signaling Pathway (469)

ncbi summary :

regulates transcription in response to low oxygen; may play a role in vascular biology [RGD, Feb 2006]

uniprot summary :

Function: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia

size1 :

48-Strip-Wells

price1 :

605 USD

size2 :

96-Strip-Wells

price2 :

870

size3 :

5x96-Strip-Wells

price3 :

2950

size4 :

10x96-Strip-Wells

price4 :

5535

more info or order :

MyBioSource product webpage