catalog number :
MBS704415
products type :
ELISA Kit
products full name :
Mouse Tumor necrosis factor beta, TNF-beta ELISA Kit
products short name :
lymphotoxin alpha (TNF superfamily, member 1)
products name syn :
Mouse Tumor necrosis factor beta; TNF-beta ELISA KIT; DAMA-25N12.13; LT; TNFB; TNFSF1; OTTHUMP00000165897; lymphotoxin alpha; tumor necrosis factor beta; lymphotoxin alpha (TNF superfamily; member 1)
other names :
lymphotoxin-alpha; Lymphotoxin-alpha; lymphotoxin-alpha; TNF beta; lymphotoxin alpha; tumor necrosis factor ligand superfamily member 1; lymphotoxin A; TNF-beta; Tumor necrosis factor ligand superfamily member 1
other gene names :
Lta; Lta; LT; Ltx; Tnfb; LT[a]; LT-[a]; TNFSF1; hlb382; LTalpha; Tnfsf1b; LT-alpha; TNF-beta; Tnfb; Tnfsf1; LT-alpha
uniprot entry name :
TNFB_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse LTA. No significant cross-reactivity or interference between Mouse LTA and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml-1200 pg/ml. Sensitivity: 4.7 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNF-beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-beta is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-beta bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_034865.1
ncbi gb acc num :
NM_010735.2
ncbi mol weight :
21,999 Da
ncbi pathways :
Apoptosis Pathway (198339); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); HTLV-I Infection Pathway (373903); HTLV-I Infection Pathway (373889); Herpes Simplex Infection Pathway (377874); Herpes Simplex Infection Pathway (377865); NF-kappa B Signaling Pathway (634537); TNF Signaling Pathway (812263); TNF Signaling Pathway (813210)
uniprot summary :
Function: Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
size4 :
10x96-Strip-Wells