product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat soluble platelet endothelial cell adhesion molecule 1, sPECAM-1 ELISA Kit
catalog :
MBS704412
quantity :
48-Strip-Wells
price :
605 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS704412
products type :
ELISA Kit
products full name :
Rat soluble platelet endothelial cell adhesion molecule 1, sPECAM-1 ELISA Kit
products short name :
platelet/endothelial cell adhesion molecule
products name syn :
Rat soluble platelet endothelial cell adhesion molecule 1; sPECAM-1 ELISA Kit; CD31; FLJ58394; PECAM-1; CD31 antigen; CD31/EndoCAM; PECAM-1; CD31/EndoCAM; adhesion molecule; platelet/endothelial cell adhesion molecule
other names :
platelet endothelial cell adhesion molecule; Platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule; PECAM-1; platelet/endothelial cell adhesion molecule 1; CD_antigen: CD31
products gene name :
PECAM1
other gene names :
Pecam1; Pecam1; CD31; Pecam; Pecam; PECAM-1
uniprot entry name :
PECA1_RAT
reactivity :
Rat
sequence length :
678
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat PECAM1. No significant cross-reactivity or interference between Rat PECAM1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml-200 ng/ml. Sensitivity: 0.78 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sPECAM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sPECAM-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sPECAM-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sPECAM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
75832033
ncbi acc num :
NP_113779.1
ncbi gb acc num :
NM_031591.1
uniprot acc num :
Q3SWT0
ncbi mol weight :
76,189 Da
ncbi pathways :
Cell Adhesion Molecules (CAMs) Pathway (83461); Cell Adhesion Molecules (CAMs) Pathway (480); Leukocyte Transendothelial Migration Pathway (83475); Leukocyte Transendothelial Migration Pathway (494); Malaria Pathway (152662); Malaria Pathway (152657)
ncbi summary :
cell adhesion molecule, also intracellular signaling molecule; may mediate leukocyte adhesion and migration, angiogenesis, and thrombosis [RGD, Feb 2006]
uniprot summary :
Function: Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-660 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Modulates BDKRB2 activation. Induces susceptibility to atherosclerosis
size1 :
48-Strip-Wells
price1 :
605 USD
size2 :
96-Strip-Wells
price2 :
870
size3 :
5x96-Strip-Wells
price3 :
2950
size4 :
10x96-Strip-Wells
price4 :
5535
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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