ELISA/assay

Human B-lymphocyte antigen, CD20 ELISA Kit

MBS704400

48-Strip-Wells

565 USD

ELISA Kit

Human B-lymphocyte antigen, CD20 ELISA Kit

membrane-spanning 4-domains, subfamily A, member 1

Human B-lymphocyte antigen (CD20) ELISA Kit; B1; Bp35; CD20; LEU-16; MGC3969; MS4A2; S7; B-lymphocyte cell-surface antigen B1; CD20 antigen; CD20 receptor; membrane-spanning 4-domains; subfamily A; member 1

B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1; membrane-spanning 4-domains, subfamily A, member 1; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD_antigen: CD20

MS4A1

MS4A1; MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16; CD20

CD20_HUMAN

Human

297

This assay has high sensitivity and excellent specificity for detection of human CD20. No significant cross-reactivity or interference between human CD20 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: The minimum detectable dose of human CD20 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD20 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD20 bound in the initial step. The color development is stopped and the intensity of the color is measured.

23110987

NP_068769.2

NM_021950.3

P11836

33,077 Da

Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489)

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

Function: This protein may be involved in the regulation of B-cell activation and proliferation.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150