product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat basic fibroblast growth factor, bFGF ELISA Kit
catalog :
MBS704396
quantity :
48-Strip-Wells
price :
465 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS704396
products type :
ELISA Kit
products full name :
Rat basic fibroblast growth factor, bFGF ELISA Kit
products short name :
fibroblast growth factor 2 (basic)
products name syn :
Rat basic fibroblast growth factor; bFGF ELISA Kit; BFGF; FGFB; HBGF-2; basic fibroblast growth factor bFGF; fibroblast growth factor 2; heparin-binding growth factor 2; prostatropin; fibroblast growth factor 2 (basic)
other names :
Fibroblast growth factor 2; Fibroblast growth factor 2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
products gene name :
FGF2
other gene names :
FGF2; FGF-2; bFGF; HBGF-2
uniprot entry name :
FGF2_RABIT
reactivity :
Rat
sequence length :
137
specificity :
This assay has high sensitivity and excellent specificity for detection of rat bFGF . No significant cross-reactivity or interference between rat bFGF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.9 pg/ml -60 pg/ml. Sensitivity: The minimum detectable dose of rat bFGF is typically less than 0.225 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for bFGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any bFGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for bFGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of bFGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
1345992
ncbi acc num :
P48799.1
uniprot acc num :
P48799
ncbi mol weight :
15,419 Da
ncbi pathways :
Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Downstream Signaling Of Activated FGFR Pathway (160957); Endochondral Ossification Pathway (198812); FGFR Ligand Binding And Activation Pathway (106344); FGFR1 Ligand Binding And Activation Pathway (106345); FGFR1b Ligand Binding And Activation Pathway (106346); FGFR1c Ligand Binding And Activation Pathway (106347); FGFR2 Ligand Binding And Activation Pathway (106349); FGFR2c Ligand Binding And Activation Pathway (106351); FGFR3 Ligand Binding And Activation Pathway (106352)
uniprot summary :
Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro
size1 :
48-Strip-Wells
price1 :
465 USD
size2 :
96-Strip-Wells
price2 :
655
size3 :
5x96-Strip-Wells
price3 :
2455
size4 :
10x96-Strip-Wells
price4 :
4595
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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