ELISA/assay

Human Interleukin 33, IL-33 ELISA Kit

MBS704373

48-Strip-Wells

565 USD

ELISA Kit

Human Interleukin 33, IL-33 ELISA Kit

interleukin 33

Human Interleukin 33 (IL-33) ELISA Kit; C9orf26; DKFZp586H0523; DVS27; NF-HEV; NFEHEV; RP11-575C20.2; DVS27-related protein; nuclear factor from high endothelial venules; interleukin 33

interleukin-33 isoform 2; Interleukin-33; interleukin-33; IL-33; IL-1F11; DVS27-related protein; interleukin-1 family member 11; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules; interleukin 33; Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules; NF-HEV

IL33

IL33; IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26; C9orf26; IL1F11; NFHEV; IL-33; IL-1F11; NF-HEV

IL33_HUMAN

Human

228

This assay has high sensitivity and excellent specificity for detection of Human IL33. No significant cross-reactivity or interference between Human IL33 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and urine. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-33. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL-33 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-33, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-33 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

313851030

NP_001186569.1

NM_001199640.1

O95760

30,759 Da

Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Influenza A Pathway (217173); Influenza A Pathway (217150)

IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011]

Function: Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (1 PublicationRef.3

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150