product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Bovine Heat Shock Protein 70, HSP-70 ELISA Kit
catalog :
MBS704371
quantity :
48-Strip-Wells
price :
565 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS704371
products type :
ELISA Kit
products full name :
Bovine Heat Shock Protein 70, HSP-70 ELISA Kit
products short name :
Heat Shock Protein 70, HSP-70
products name syn :
Bovine Heat Shock Protein 70; HSP-70 ELISA Kit; Heat Shock Protein 70; HSP-70
other names :
HSP70; HSP70; HSP70
products gene name :
HSP-70
uniprot entry name :
Q40058_HORVU
reactivity :
Bovine
sequence length :
608
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine HSP-70. No significant cross-reactivity or interference between bovine HSP-70 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 1.2 5 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of bovine HSP-70 is typically less than 0.63 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest bovine HSP-70 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP-70. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated HSP-70. A competitive inhibition reaction is launched between HSP-70 (Standards or samples) and Biotin-conjugated HSP-70 with the pre-coated antibody specific for HSP-70. The more amount of HSP-70 in samples, the less antibody bound by Biotin-conjugated HSP-70. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of HSP-70 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
476003
ncbi acc num :
AAA62325.1
uniprot acc num :
Q40058
ncbi mol weight :
67,016 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size1 :
48-Strip-Wells
price1 :
565 USD
size2 :
96-Strip-Wells
price2 :
810
size3 :
5x96-Strip-Wells
price3 :
2750
size4 :
10x96-Strip-Wells
price4 :
5150
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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