Fish Tumor Necrosis Factor alpha, TNF-alpha ELISA Kit | MyBioSource MBS704369 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Fish Tumor Necrosis Factor alpha, TNF-alpha ELISA Kit

catalog :

MBS704369

quantity :

48-Strip-Wells

price :

590 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704369

products type :

ELISA Kit

products full name :

Fish Tumor Necrosis Factor alpha, TNF-alpha ELISA Kit

products short name :

Tumor Necrosis Factor alpha (TNF-alpha)

products name syn :

Fish Tumor Necrosis Factor alpha (TNF-alpha) ELISA KIT; Tumor Necrosis Factor alpha (TNF-alpha)

other names :

TNF-alpha; Tumor necrosis factor; tumor necrosis factor; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

products gene name :

TNF-alpha

other gene names :

TNF; TNF; DIF; TNFA; TNFSF2; TNF-alpha; TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2

uniprot entry name :

TNFA_HUMAN

reactivity :

Fish

sequence length :

233

specificity :

This assay has high sensitivity and excellent specificity for detection of fish TNF-alpha. No significant cross-reactivity or interference between fish TNF-alpha and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma and tissue homogenates. Assay Type: Competitive. Detection Range: 192 pg/ml-5000 pg/ml. Sensitivity: The minimum detectable dose of fish TNF-alpha is typically less than 192 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest fish TNF-alpha concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for TNF-alpha and Horseradish Peroxidase (HRP) conjugated TNF-alpha. The competitive inhibition reaction is launched between with HRP labeled TNF-alpha and unlabeled TNF-alpha with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TNF-alpha in the sample. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

37210

ncbi acc num :

CAA26669.1

uniprot acc num :

P01375

ncbi mol weight :

25,644 Da

ncbi pathways :

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); AhR Pathway (755436); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Alzheimer's Disease Pathway (83097)

ncbi summary :

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.1 PublicationManual assertion based on experiment in:Ref.24

size1 :

48-Strip-Wells

price1 :

590 USD

size2 :

96-Strip-Wells

price2 :

850

size3 :

5x96-Strip-Wells

price3 :

2880

size4 :

10x96-Strip-Wells

price4 :

5440

more info or order :

MyBioSource product webpage