Human Tissue Inhibitor Of Matrix Metalloprotease-1, TIMP-1 ELISA Kit | MyBioSource MBS704361 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Tissue Inhibitor Of Matrix Metalloprotease-1, TIMP-1 ELISA Kit

catalog :

MBS704361

quantity :

96-Strip-Wells

price :

630 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704361

products type :

ELISA Kit

products full name :

Human Tissue Inhibitor Of Matrix Metalloprotease-1, TIMP-1 ELISA Kit

products short name :

TIMP metallopeptidase inhibitor 1

products name syn :

Human Tissue Inhibitor Of Matrix Metalloprotease-1 (TIMP-1) ELISA KIT; RP1-230G1.3; CLGI; EPA; EPO; FLJ90373; HCI; TIMP; erythroid potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinase 1; Human Tissue Inhibitor Of Matrix Metalloprotease-1 (TIMP-1)

other names :

metalloproteinase inhibitor 1; Metalloproteinase inhibitor 1; metalloproteinase inhibitor 1; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1; TIMP metallopeptidase inhibitor 1; Erythroid-potentiating activity; EPA; Fibroblast collagenase inhibitor; Collagenase inhibitor; Tissue inhibitor of metalloproteinases 1; TIMP-1

products gene name :

TIMP1

other gene names :

TIMP1; TIMP1; EPA; EPO; HCI; CLGI; TIMP; CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1

uniprot entry name :

TIMP1_HUMAN

reactivity :

Human

sequence length :

207

specificity :

This assay has high sensitivity and excellent specificity for detection of Human TIMP1. No significant cross-reactivity or interference between Human TIMP1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 0.39 ng/ml-25 ng/ml. Sensitivity: 0.434 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cardiovascular

products description :

This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to TIMP-1 has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated TIMP-1 and incubated. A competitive inhibition reaction is launched between TIMP-1 (Standards or samples) and HRP-conjugated TIMP-1 with the pre-coated antibody specific for TIMP-1. The more amount of TIMP-1 in samples, the less antibody bound by HRP-conjugated TIMP-1. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of TIMP-1 in the sample. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4507509

ncbi acc num :

NP_003245.1

ncbi gb acc num :

NM_003254.2

uniprot acc num :

P01033

ncbi mol weight :

23,171 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); HIF-1 Signaling Pathway (695200); Hemostasis Pathway (106028); IL6-mediated Signaling Events Pathway (137932); Matrix Metalloproteinases Pathway (198900); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)

ncbi summary :

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.12 PublicationsManual assertion based on experiment in:Ref.1

size1 :

96-Strip-Wells

price1 :

630 USD

size2 :

5x96-Strip-Wells

price2 :

1955

size3 :

10x96-Strip-Wells

price3 :

3635

more info or order :

MyBioSource product webpage