ELISA/assay

Rat neutrophil elastase, NE ELISA Kit

MBS704356

96 Strip Wells

895 USD

ELISA Kit

Rat neutrophil elastase, NE ELISA Kit

neutrophil elastase, NE

Rat neutrophil elastase; NE ELISA Kit; neutrophil elastase; NE

neutrophil elastase preproprotein; Neutrophil elastase; neutrophil elastase; elastase-2; medullasin; PMN elastase; leukocyte elastase; elastase 2, neutrophil; human leukocyte elastase; polymorphonuclear elastase; bone marrow serine protease; granulocyte-derived elastase; elastase, neutrophil expressed; Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase

NE

ELANE; ELANE; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E; ELA2; HLE

ELNE_HUMAN

Rat

267

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, cell culture supernates, tissue homogenates, cell lysates. Detection Range: 12.5 ng/ml-800 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 3.12 ng/ml

Sample Volume: 50-100ul

Introduction: Neutrophil elastase is a serine protease in the same family as chymotrypsin and has broad substrate specificity. Secreted by neutrophils during inflammation, it destroys bacteria and host tissue. As with other serine proteases it contains a charge relay system composed of the catalytic triad of histidine, aspartate, and serine residues that are dispersed throughout the primary sequence of the polypeptide but that are brought together in the three dimension conformation of the folded protein. The gene encoding neutrophil elastase consists of five exons. It is one of the two forms of elastase. The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate side chains and four intramolecular disulfide bridges. It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb. Neutrophil elastase is an important protease enzyme that when expressed aberrantly can cause emphysema or emphysematous changes. This involves breakdown of the lung structure and increased airspaces. Mutations of the NE gene cause severe congenital neutropenia, which is a failure of neutrophils to mature. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NE and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4503549

NP_001963.1

NM_001972.2

P08246

28,518 Da

Activation Of Matrix Metalloproteinases Pathway 576264!!C-MYB Transcription Factor Network Pathway 138073!!Collagen Degradation Pathway 730309!!Degradation Of The Extracellular Matrix Pathway 576263!!Extracellular Matrix Organization Pathway 576262!!Systemic Lupus Erythematosus Pathway 83122!!Systemic Lupus Erythematosus Pathway 534!!Transcriptional Misregulation In Cancer Pathway 523016!!Transcriptional Misregulation In Cancer Pathway 522987!!Amb2 Integrin Signaling Pathway 137945

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]

Function: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.1 PublicationManual assertion based on experiment in:Ref.15. Catalytic activity: Hydrolysis of proteins, including elastin. Preferential cleavage: Val- -Xaa > Ala- -Xaa.

96 Strip Wells

895 USD