ELISA/assay

Human lipolysaccharide binding protein, LBP ELISA Kit

MBS704355

48-Strip-Wells

565 USD

ELISA Kit

Human lipolysaccharide binding protein, LBP ELISA Kit

lipopolysaccharide binding protein

Human lipolysaccharide binding protein; LBP ELISA Kit; MGC22233; LPS-binding protein; lipopolysaccharide-binding protein; lipopolysaccharide binding protein

lipopolysaccharide-binding protein; Lipopolysaccharide-binding protein; lipopolysaccharide-binding protein; LPS-binding protein; BPI fold containing family D, member 2; lipopolysaccharide binding protein

LBP

LBP; LBP; BPIFD2; LBP

LBP_HUMAN

Human

481

This assay has high sensitivity and excellent specificity for detection of Human LBP. No significant cross-reactivity or interference between Human LBP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ug/ml-40 ug/ml. Sensitivity: 0.156 ug/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 3: Lipid transport

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

31652249

NP_004130.2

NM_004139.4

P18428

53,384 Da

IL6-mediated Signaling Events Pathway (137932); Immune System Pathway (106386); Innate Immune System Pathway (106387); NF-kappa B Signaling Pathway (634527); Regulation Of Toll-like Receptor Signaling Pathway (920955); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149); Toll Like Receptor 4 (TLR4) Cascade Pathway (106398); Toll-Like Receptors Cascades Pathway (106388); Toll-like Receptor Signaling Pathway (83076)

The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). [provided by RefSeq, Apr 2012]

Function: Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.2 PublicationsManual assertion based on experiment in:Ref.10

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150