Sheep neutrophil gelatinase-associated lipocalin, NGAL ELISA Kit | MyBioSource MBS704353 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Sheep neutrophil gelatinase-associated lipocalin, NGAL ELISA Kit

catalog :

MBS704353

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704353

products type :

ELISA Kit

products full name :

Sheep neutrophil gelatinase-associated lipocalin, NGAL ELISA Kit

products short name :

neutrophil gelatinase-associated lipocalin, NGAL

products name syn :

Sheep neutrophil gelatinase-associated lipocalin; NGAL ELISA Kit; neutrophil gelatinase-associated lipocalin; NGAL

other names :

neutrophil gelatinase-associated lipocalin; Neutrophil gelatinase-associated lipocalin; neutrophil gelatinase-associated lipocalin; p25; lipocalin-2; oncogene 24p3; siderocalin LCN2; migration-stimulating factor inhibitor; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; lipocalin 2; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25

products gene name :

NGAL

other gene names :

LCN2; LCN2; 24p3; MSFI; NGAL; HNL; NGAL; NGAL

uniprot entry name :

NGAL_HUMAN

reactivity :

Sheep

sequence length :

198

specificity :

This assay has high sensitivity and excellent specificity for detection of sheep NGAL. No significant cross-reactivity or interference between sheep NGAL and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, and tissue homogenates. Assay Type: Competitive. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of sheep NGAL is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NGAL. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for NGAL. The competitive inhibition reaction is launched between with pre-coated NGAL and NGAL in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of NGAL in the samples. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

38455402

ncbi acc num :

NP_005555.2

ncbi gb acc num :

NM_005564.3

uniprot acc num :

P80188

ncbi mol weight :

22,457 Da

uniprot summary :

Function: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.1 PublicationManual assertion based on experiment in:Ref.11

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage