ELISA/assay

Fish Insulin, INS ELISA Kit

MBS704336

96 Strip Wells

915 USD

ELISA Kit

Fish Insulin, INS ELISA Kit

Insulin, INS

Fish Insulin; INS ELISA Kit; Insulin; INS

insulin; Insulin; insulin; proinsulin; preproinsulin; insulin-dependent diabetes mellitus 2; insulin

INS

INS; INS; ILPR; IRDN; IDDM1; IDDM2; MODY10

INS_HUMAN

Fish

110

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, tissue homogenates. Detection Range: 100-1600pg/ml. Detection Wavelength: 450 nm. Sensitivity: 50 pg/ml

Sample Volume: 50-100ul

Introduction: Insulin is a peptide hormone composed of 51 amino acid residues and has a molecular weight of 5808 Da. It is produced in the islets of Langerhans in the pancreas. Insulin's structure varies slightly between species of animal. Insulin has extensive effects on both metabolism and several other body systems (eg, vascular compliance). Insulin causes most of the body's cells to take up glucose from the blood (including liver, muscle, and fat tissue cells), storing it as glycogen in the liver and muscle, and stops use of fat as an energy source. When insulin is absent (or low), glucose is not taken up by most body cells and the body begins to use fat as an energy source (ie, transfer of lipids from adipose tissue to the liver for mobilization as an energy source). As its level is a central metabolic control mechanism, its status is also used as a control signal to other body systems (such as amino acid uptake by body cells). It has several other anabolic effects throughout the body. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A antibody specific to INS has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated INS and incubated. A competitive inhibition reaction is launched between INS (Standards or samples) and Biotin-conjugated INS with the pre-coated antibody specific for INS. The more amount of INS in samples, the less antibody bound by Biotin-conjugated INS. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of INS in the sample. The color development is stopped and the intensity of the color is measured.

386828

AAA59172.1

P01308

21,537 Da

AGE/RAGE Pathway 698754!!AMPK Signaling Pathway 989139!!AMPK Signaling Pathway 992181!!ATF-2 Transcription Factor Network Pathway 138006!!Adipogenesis Pathway 198832!!Aldosterone-regulated Sodium Reabsorption Pathway 130626!!Aldosterone-regulated Sodium Reabsorption Pathway 130590!!Amyloids Pathway 366238!!Arf6 Trafficking Events Pathway 137954!!Cardiac Progenitor Differentiation Pathway 712094

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Function: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

96 Strip Wells

915 USD