Human Interleukin 18, IL-18 ELISA Kit | MyBioSource MBS704332 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Interleukin 18, IL-18 ELISA Kit

catalog :

MBS704332

quantity :

96-Strip-Wells

price :

630 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704332

products type :

ELISA Kit

products full name :

Human Interleukin 18, IL-18 ELISA Kit

products short name :

interleukin 18 (interferon-gamma-inducing factor)

products name syn :

Human Interleukin 18; IL-18 ELISA kit; IGIF; IL-18; IL-1g; IL1F4; MGC12320; IL-1 gamma; interferon-gamma-inducing factor; interleukin 18; interleukin-1 gamma; interleukin-18; Human Interleukin 18; IL-18

other names :

interleukin-18 isoform 2 proprotein; Interleukin-18; interleukin-18; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); interleukin 18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma

products gene name :

IL18

other gene names :

IL18; IL18; IGIF; IL-18; IL-1g; IL1F4; IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma

uniprot entry name :

IL18_HUMAN

reactivity :

Human

sequence length :

189

specificity :

This assay has high sensitivity and excellent specificity for detection of Human IL18. No significant cross-reactivity or interference between Human IL18 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, urine, tissue homogenates. Detection Range: 7.8 pg/ml-500 pg/ml. Sensitivity: 1.95 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cytokine

products description :

Introduction: Interleukin-18 also known as IL18 is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine. IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. IL-18 works together with IL-12 to induce cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-gamma (IFN-gamma) or type II interferon that plays an important role in activating the macrophages or other cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-18. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL-18 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-18 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

342349321

ncbi acc num :

NP_001230140.1

ncbi gb acc num :

NM_001243211.1

uniprot acc num :

Q14116

ncbi mol weight :

22,326 Da

ncbi pathways :

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Cellular Roles Of Anthrax Toxin Pathway (138076); Corticotropin-releasing Hormone Pathway (920957); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Hypertrophy Model Pathway (198828)

ncbi summary :

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

uniprot summary :

Function: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

size1 :

96-Strip-Wells

price1 :

630 USD

size2 :

5x96-Strip-Wells

price2 :

1955

size3 :

10x96-Strip-Wells

price3 :

3635

more info or order :

MyBioSource product webpage