ELISA/assay

Bovine Interferon gamma, IFN-gamma ELISA Kit

MBS704330

48-Strip-Wells

465 USD

ELISA Kit

Bovine Interferon gamma, IFN-gamma ELISA Kit

interferon, gamma

Bovine Interferon gamma; IFN-gamma ELISA Kit; IFG; IFI; ; interferon; gamma

interferon gamma; Interferon gamma; interferon gamma; boIFNG; IFN-gamma; interferon-gamma; interferon gamma type 2; interferon, gamma

IFNG

IFNG; IFNG; BoIFNG; IFN-gamma

IFNG_BOVIN

Bovine

166

This assay has high sensitivity and excellent specificity for detection of bovine IFN -gamma. No significant cross-reactivity or interference between bovine IFN -gamma and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of bovine IFN -gamma is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFN-gamma has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-gamma present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-gamma is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-gamma bound in the initial step. The color development is stopped and the intensity of the color is measured.

31982949

NP_776511.1

NM_174086.1

P07353

19,393 Da

African Trypanosomiasis Pathway (194381); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (84358); Allograft Rejection Pathway (535); Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Antigen Processing And Presentation Pathway (84313); Antigen Processing And Presentation Pathway (485); Chagas Disease (American Trypanosomiasis) Pathway (147808); Chagas Disease (American Trypanosomiasis) Pathway (147795)

Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

48-Strip-Wells

465 USD

96-Strip-Wells

655

5x96-Strip-Wells

2455

10x96-Strip-Wells

4595