ELISA/assay

Rat L-Lactate Dehydrogenase, L-LDH ELISA Kit

MBS704326

96 Strip Wells

850 USD

ELISA Kit

Rat L-Lactate Dehydrogenase, L-LDH ELISA Kit

lactate dehydrogenase A

Rat L-Lactate Dehydrogenase; L-LDH ELISA Kit; GSD11; LDH1; LDHM; PIG19; LDH muscle subunit; lactate dehydrogenase M; proliferation-inducing gene 19; renal carcinoma antigen NY-REN-59; lactate dehydrogenase A

L-lactate dehydrogenase A chain; L-lactate dehydrogenase A chain; L-lactate dehydrogenase A chain; LDH-A; LDH muscle subunit; lactate dehydrogenase-M; LDH muscle subunit; LDH-M

LDHA

LDHA; LDHA; LDH-M; LDH-A; LDH-M

LDHA_RABIT

Rat

332

This assay has high sensitivity and excellent specificity for detection of Rat LDHA. No significant cross-reactivity or interference between Rat LDHA and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 39 mU/ml-2500 mU/ml. Detection Wavelength: 450 nm. Sensitivity: 9.7 mU/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: glyconeogenesis and glycometabolism. Protein Biological Process 3: Glycolysis

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to L-LDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for L-LDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain L-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of L-LDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

126722813

NP_001075746.1

NM_001082277.1

P13491

36,565 Da

Biosynthesis Of Secondary Metabolites Pathway 212418!!Cysteine And Methionine Metabolism Pathway 212373!!Cysteine And Methionine Metabolism Pathway 103421!!Glycolysis / Gluconeogenesis Pathway 212361!!Glycolysis / Gluconeogenesis Pathway 287!!Metabolic Pathways 212413!!Microbial Metabolism In Diverse Environments Pathway 212417!!Propanoate Metabolism Pathway 212399!!Propanoate Metabolism Pathway 387!!Pyruvate Metabolism Pathway 212397

96 Strip Wells

850 USD