Human A Disintegrin And Metalloprotease 10, ADAM10 ELISA Kit | MyBioSource MBS704324 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human A Disintegrin And Metalloprotease 10, ADAM10 ELISA Kit

catalog :

MBS704324

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704324

products type :

ELISA Kit

products full name :

Human A Disintegrin And Metalloprotease 10, ADAM10 ELISA Kit

products short name :

ADAM metallopeptidase domain 10

products name syn :

Human A Disintegrin And Metalloprotease 10; ADAM10 ELISA Kit; AD10; CD156c; HsT18717; MADM; kuz; a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10; kuzbanian; ADAM metallopeptidase domain 10

other names :

disintegrin and metalloproteinase domain-containing protein 10; Disintegrin and metalloproteinase domain-containing protein 10; disintegrin and metalloproteinase domain-containing protein 10; CDw156; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10; ADAM metallopeptidase domain 10; CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; CD_antigen: CD156c

products gene name :

ADAM10

other gene names :

ADAM10; ADAM10; RAK; kuz; AD10; AD18; MADM; CD156c; HsT18717; KUZ; MADM; ADAM 10

uniprot entry name :

ADA10_HUMAN

reactivity :

Human

sequence length :

748

specificity :

This assay has high sensitivity and excellent specificity for detection of human ADAM10. No significant cross-reactivity or interference between human ADAM10 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: 1.95 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAM10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAM10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4557251

ncbi acc num :

NP_001101.1

ncbi gb acc num :

NM_001110.3

uniprot acc num :

O14672

ncbi mol weight :

84,142 Da

ncbi pathways :

Activated NOTCH1 Transmits Signal To The Nucleus Pathway (576270); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Axon Guidance Pathway (105688); Collagen Degradation Pathway (730309); Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway (771591); Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (771595); Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway (771593); Constitutive Signaling By NOTCH1 T(7;9)(NOTCH1:M1580_K2555) Translocation Mutant Pathway (771589)

ncbi summary :

Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala- -Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.8 PublicationsManual assertion based on experiment in:Ref.4. Catalytic activity: Endopeptidase of broad specificity. Cofactor: Binds 1 zinc ion

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage