ELISA/assay

Rat Granulocyte Colony Stimulating Factor, G-CSF ELISA Kit

MBS704320

48-Strip-Wells

465 USD

ELISA Kit

Rat Granulocyte Colony Stimulating Factor, G-CSF ELISA Kit

Granulocyte Colony Stimulating Factor, G-CSF

Rat Granulocyte Colony Stimulating Factor; G-CSF ELISA Kit; Granulocyte Colony Stimulating Factor; G-CSF

granulocyte colony-stimulating factor, partial; Granulocyte colony-stimulating factor; granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin; colony stimulating factor 3 (granulocyte); Pluripoietin; INN: Filgrastim; INN: Lenograstim

G-CSF

CSF3; CSF3; GCSF; CSF3OS; C17orf33; C17orf33; GCSF; G-CSF

CSF3_HUMAN

Rat

138

This assay has high sensitivity and excellent specificity for detection of rat G-CSF. No significant cross-reactivity or interference between rat G-CSF and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.81 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for G-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any G-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for G-CSF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.

297660573

ADI49832.1

P09919

18,434 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Malaria Pathway (152665); Malaria Pathway (152657); PI3K-Akt Signaling Pathway (692234)

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

48-Strip-Wells

465 USD

96-Strip-Wells

655

5x96-Strip-Wells

2455

10x96-Strip-Wells

4595