ELISA/assay

Human thymus activation regulated chemokine, TARC ELISA Kit

MBS704319

96 Strip Wells

550 USD

ELISA Kit

Human thymus activation regulated chemokine, TARC ELISA Kit

chemokine (C-C motif) ligand 17

Human thymus activation regulated chemokine; TARC ELISA Kit; A-152E5.3; ABCD-2; MGC138271; MGC138273; SCYA17; TARC; T cell-directed CC chemokine; small inducible cytokine A17; small inducible cytokine subfamily A (Cys-Cys); member 17; thymus and activation-regul; chemokine (C-C motif) ligand 17

C-C motif chemokine 17; C-C motif chemokine 17; C-C motif chemokine 17; CC chemokine TARC; T cell-directed CC chemokine; small-inducible cytokine A17; thymus and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 17; chemokine (C-C motif) ligand 17; CC chemokine TARC; Small-inducible cytokine A17; Thymus and activation-regulated chemokine

CCL17

CCL17; CCL17; TARC; ABCD-2; SCYA17; A-152E5.3; SCYA17; TARC

CCL17_HUMAN

Human

94

This assay has high sensitivity and excellent specificity for detection of human TARC. No significant cross-reactivity or interference between human TARC and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human thymus activation regulated chemokine (TARC) concentrations in serum, plasma, tissue homogenates. Detection Range: 3.12 pg/ml -200 pg/ml.

Sensitivity: The minimum detectable dose of human TARC is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: </b<This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TARC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TARC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TARC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TARC bound in the initial step. The color development is stopped and the intensity of the color is measured.

4506829

NP_002978.1

NM_002987.2

Q92583

10,507 Da

Chemokine Receptors Bind Chemokines Pathway 106359!!Chemokine Signaling Pathway 99051!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!GPCR Ligand Binding Pathway 161020!!IL4-mediated Signaling Events Pathway 137933!!Peptide Ligand-binding Receptors Pathway 106358!!Signal Transduction Pathway 477114

This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for T lymphocytes, but not monocytes or granulocytes. The product of this gene binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells. [provided by RefSeq, Jul 2008]

Function: Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4.1 PublicationManual assertion based on experiment in:Ref.7

96 Strip Wells

550 USD