ELISA/assay

Human Prostaglandin F2alpha, PGF2alpha ELISA Kit

MBS704294

96 Strip Wells

575 USD

ELISA Kit

Human Prostaglandin F2alpha, PGF2alpha ELISA Kit

Prostaglandin F2alpha, PGF2alpha

Human Prostaglandin F2alpha; PGF2alpha ELISA Kit; Prostaglandin F2alpha; PGF2alpha

PGF2alpha

Human

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, tissue homogenates. Detection Range: 1.56 pg/ml-100 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 0.39 pg/ml

Sample Volume: 50-100ul

Introduction: Prostaglandin F2alpha (PGF2alpha) is pharmaceutically termed Dinoprost , is a naturally-occurring prostaglandin used in medicine to induce labor and as an abortifacient. In domestic mammals, it is produced by the uterus when stimulated by oxytocin, in the event that there has been no implantation during the follicular phase. It acts on the corpus luteum to cause luteolysis, forming a corpus albicans and stopping the production of progesterone. Action of PGF2alpha is dependent on the number of receptors on the corpus luteum membrane. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PGF2alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PGF2alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PGF2alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PGF2alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96 Strip Wells

575 USD