ELISA/assay

Rat N-terminal procollagen III propeptide, PIIINP ELISA Kit

MBS704287

96 Strip Wells

915 USD

ELISA Kit

Rat N-terminal procollagen III propeptide, PIIINP ELISA Kit

N-terminal procollagen III propeptide, PIIINP

Rat N-terminal procollagen III propeptide; PIIINP ELISA Kit; N-terminal procollagen III propeptide; PIIINP

PIIINP

Rat

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.625 ng/ml-40 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.156 ng/ml

Sample Volume: 50-100ul

Introduction: Collagens are the major fibrillar components of most connective tissues. At least nineteen different subtypes of collagens have been identified. The collagens I, III, IV, V and VI are known to predominate in normal and fibrotic lungs. Collagens form a family of proteins, and each type has its own characteristic amino acid sequence. Interstitial collagens are synthesised and secreted out of cells as procollagens. During the secretion, carboxy- and aminoterminal propeptides are cleaved off from the parent molecule by specific proteases. This releases the collagen molecule, together with its respective procollagen propeptides, in stoichiometric amounts into the extracellular space. The propeptide molecules are then further degraded by non-specific proteases to smaller peptides, which retain the antigenicity of the parent procollagen propeptide molecule. The cleavage of the aminoterminal propeptide can be incomplete, resulting in the formation of a collagen molecule with attached aminoterminal propeptide (pN-collagen). Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PIIINP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PIIINP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PIIINP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PIIINP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96 Strip Wells

915 USD