ELISA/assay

Human Chromogranin, CgA ELISA Kit

MBS704285

48-Strip-Wells

565 USD

ELISA Kit

Human Chromogranin, CgA ELISA Kit

chromogranin A (parathyroid secretory protein 1)

Human Chromogranin; CgA ELISA Kit; CGA; betagranin (N-terminal fragment of chromogranin A) ; chromogranin A; parathyroid secretory protein 1; chromogranin A (parathyroid secretory protein 1)

chromogranin-A preproprotein; Chromogranin-A; chromogranin-A; SP-I; pituitary secretory protein I; parathyroid secretory protein 1; betagranin (N-terminal fragment of chromogranin A); chromogranin A (parathyroid secretory protein 1); Pituitary secretory protein I; SP-I

CHGA

CHGA; CHGA; CGA; CgA; SP-I

CMGA_HUMAN

Human

457

This assay has high sensitivity and excellent specificity for detection of human CgA. No significant cross-reactivity or interference between human CgA and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, urine, cerebrospinal fluid (CSF). Assay Type: Sandwich. Detection Range: 62.5 ng/ml -4000 ng/ml. Sensitivity: 15.6 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CgA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CgA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CgA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CgA bound in the initial step. The color development is stopped and the intensity of the color is measured.

4502805

NP_001266.1

NM_001275.3

P10645

50,688 Da

Amine-derived Hormones Pathway (160983); Androgen Biosynthesis Pathway (106154); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Glucocorticoid Receptor Regulatory Network Pathway (138014); Glycoprotein Hormones Pathway (160989)

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Function: Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150