catalog number :
MBS704277
products type :
ELISA Kit
products full name :
Human Immunoglobulin J chain, IGJ ELISA Kit
products short name :
immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides
products name syn :
Human Immunoglobulin J chain (IGJ) ELISA kit; IGCJ; JCH; immunoglobulin J chain; immunoglobulin J polypeptide; linker protein for immunoglobulin alpha and mu polypeptides
other names :
immunoglobulin J chain; Immunoglobulin J chain; immunoglobulin J chain; IgJ chain; immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides
other gene names :
IGJ; IGJ; JCH; IGCJ; IGCJ
uniprot entry name :
IGJ_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human IGJ. No significant cross-reactivity or interference between human IGJ and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, saliva aliva aliva, urine, tear, breast milk. Assay Type: Sandwich. Detection Range: 10ng/ml -40000 ng/ml. Sensitivity: 10 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGJ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGJ present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for IGJ is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGJ bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_653247.1
ncbi gb acc num :
NM_144646.3
ncbi mol weight :
18,099 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.
size4 :
10x96-Strip-Wells