catalog number :
MBS704262
products type :
ELISA Kit
products full name :
Mouse Vascular Endothelial cell Growth Factor B, VEGF-B ELISA Kit
products short name :
vascular endothelial growth factor B
products name syn :
Mouse Vascular Endothelial cell Growth Factor B; VEGF-B ELISA kit; VEGFL; VRF; ; vascular endothelial growth factor B
other names :
vascular endothelial growth factor B isoform Vegf-b167; Vascular endothelial growth factor B; vascular endothelial growth factor B; VEGF-related factor; vascular endothelial growth factor B; VEGF-related factor; VRF
products gene name :
VEGFB
other gene names :
Vegfb; Vegfb; Vrf; VEGF-B; Vrf; VEGF-B; VRF
uniprot entry name :
VEGFB_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse VEGFB. No significant cross-reactivity or interference between Mouse VEGFB and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml-500 pg/ml. Sensitivity: 1.95 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Angiogenesis. Protein Biological Process 3: Angiogenesis
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGF-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF-B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF-B bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001172093.1
ncbi gb acc num :
NM_001185164.1
ncbi mol weight :
21,914 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); Heart Development Pathway (672437); Hemostasis Pathway (1001295); PI3K-Akt Signaling Pathway (692242); PI3K-Akt Signaling Pathway (692979); PI3K/Akt/mTOR Signaling Pathway (522982)
uniprot summary :
Function: Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. VEGF-B seems to be required for normal heart function in adult but is not required for proper development of the cardiovascular system either during development or for angiogenesis in adults.1 PublicationManual assertion based on experiment in:Ref.6
size4 :
10x96-Strip-Wells