ELISA/assay

Human transient receptor potential Cation channel subfamily V member 1, TrpV1 ELISA Kit

MBS704253

48-Strip-Wells

565 USD

ELISA Kit

Human transient receptor potential Cation channel subfamily V member 1, TrpV1 ELISA Kit

transient receptor potential cation channel, subfamily V, member 1

Human transient receptor potential cation channel subfamily V member 1 (TrpV1) ELISA Kit; DKFZp434K0220; VR1; capsaicin receptor; transient receptor potential vanilloid 1a; transient receptor potential vanilloid 1b; vanilloid receptor subtype 1; transient receptor potential cation channel; subfamily V; member 1

transient receptor potential cation channel subfamily V member 1; Transient receptor potential cation channel subfamily V member 1; Capsaicin receptor; Osm-9-like TRP channel 1; OTRPC1; Vanilloid receptor 1

TRPV1

TRPV1; VR1; TrpV1; OTRPC1

TRPV1_HUMAN

Human

839

This assay has high sensitivity and excellent specificity for detection of human TrpV1. No significant cross-reactivity or interference between human TrpV1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of human TrpV1 is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TrpV1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TrpV1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TrpV1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TrpV1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

74315350

NP_061197.4

NM_018727.5

Q8NER1

94,956 Da

Inflammatory Mediator Regulation Of TRP Channels Pathway (948277); Inflammatory Mediator Regulation Of TRP Channels Pathway (948307); Ion Channel Transport Pathway (187193); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Stimuli-sensing Channels Pathway (771568); TRP Channels Pathway (906028); Transmembrane Transport Of Small Molecules Pathway (106572); Trk Receptor Signaling Mediated By PI3K And PLC-gamma Pathway (137967); Trk Receptor Signaling Mediated By The MAPK Pathway (138044)

Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008]

Function: Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca2+-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH 6) directly activate the channel.4 PublicationsManual assertion based on experiment in:Ref.1. Enzyme regulation: Channel activity is activated via the interaction with PIRT and phosphatidylinositol 4,5-bisphosphate (PIP2). Both PIRT and PIP2 are required to activate channel activity The channel is sensitized by ATP binding. Repeated stimulation with capsaicin gives rise to progressively smaller responses, due to desensitization. This desensitization is triggered by the influx of calcium ions and is inhibited by elevated ATP levels. Ca2+ and CALM displace ATP from its binding site and trigger a conformation change that leads to a closed, desensitized channel. Intracellular PIP2 inhibits desensitization. The double-knot toxin (DkTx) from the Chinese earth tiger tarantula activates the channel and traps it in an open conformation

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150