ELISA/assay

Human nuclear factor- degree B p65, NF- degree B p65 ELISA Kit

MBS704252

96 Strip Wells

850 USD

ELISA Kit

Human nuclear factor- degree B p65, NF- degree B p65 ELISA Kit

v-rel reticuloendotheliosis viral oncogene homolog A (avian)

Human nuclear factor-kappaB p65; NF-kappaB p65 ELISA Kit; MGC131774; NFKB3; p65; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene; v-rel reticuloendotheliosis viral oncogene homolog A (avian)

transcription factor p65 isoform 2; Transcription factor p65; transcription factor p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; v-rel reticuloendotheliosis viral oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; v-rel avian reticuloendotheliosis viral oncogene homolog A; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

RELA

RELA; RELA; p65; NFKB3; NFKB3

TF65_HUMAN

Human

548

This assay has high sensitivity and excellent specificity for detection of Human RELA. No significant cross-reactivity or interference between Human RELA and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma and tissue homogenates. Detection Range: 46.88 pg/ml -3000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 11.72 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Host-virus interaction

Introduction: NFkB (Nuclear Factor NF-kappa-B) is a pleiotropic transcription factor that plays a role in many biological processes, including inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. It is found as a homo- or heterodimeric complex containing the Rel-like domain containing proteins NFkB p65 (RELA/p65), RELB, NFkB1/p105, NFkB1/p50, REL and NFkB2/p52. The heterodimeric NFkB p65/p50 complex is the most abundant one. The dimers bind to kappa-B sites at their target genes, with the affinity of the interaction dependent on the subunit composition of the dimer. Furthermore, different dimers act as transcriptional activators or repressors, with the NFkB p65/p50 and p65-c-Rel complexes acting as activators. NFkB activity is controlled by several different mechanisms, including post-translational modifications, subcellular localisation and interactions with other coactivators or corepressors. NFkB complexes are held in the cytoplasm in an inactive state by interaction with members of the NFkB inhibitor (IkB) family. Typically, phosphorylation of IkB by IkB kinases (IKKs) in response to different activators leads to degradation of the inhibitor, allowing NFkB to translocate into the nucleus. The inhibitory effect of IkBs is primarily exerted through their interaction with NfKB p65. NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NF-kappaB p65. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NF-kappaB p65 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NF-kappaB p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NF-kappaB p65 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

223468681

NP_001138610.1

NM_001145138.1

Q04206

60,219 Da

AGE/RAGE Pathway 698754!!Activated TLR4 Signalling Pathway 106400!!Activation Of NF-kappaB In B Cells Pathway 576251!!Acute Myeloid Leukemia Pathway 83117!!Acute Myeloid Leukemia Pathway 529!!Adaptive Immune System Pathway 366160!!Adipocytokine Signaling Pathway 83093!!Adipocytokine Signaling Pathway 505!!Amoebiasis Pathway 167324!!Amoebiasis Pathway 167191

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Function: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.7 PublicationsManual assertion based on experiment in:Ref.15

96 Strip Wells

850 USD