catalog number :
MBS704247
products type :
ELISA Kit
products full name :
Mouse trypsin ELISA Kit
products short name :
trypsin
products name syn :
Mouse trypsin ELISA Kit; trypsin
other names :
trypsin; Trypsin-1; Antryp1
products gene name :
trypsin
other gene names :
TRYP1; AGAP008296
uniprot entry name :
TRY1_ANOGA
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse trypsin. No significant cross-reactivity or interference between mouse trypsin and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: 0.39 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for trypsin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any trypsin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for trypsin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of trypsin bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
CAA80512.1
ncbi mol weight :
29,057 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Major function may be to aid in digestion of the blood meal.2 PublicationsManual assertion based on experiment in:Ref.1. Catalytic activity: Preferential cleavage: Arg- -Xaa, Lys- -Xaa.
size4 :
10x96-Strip-Wells