ELISA/assay

Mouse Pulmonary Surfactant-associated protein B, SP-B ELISA Kit

MBS704231

48-Strip-Wells

510 USD

ELISA Kit

Mouse Pulmonary Surfactant-associated protein B, SP-B ELISA Kit

surfactant protein B

Mouse Pulmonary Surfactant-associated protein B (SP-B) ELISA kit; PSP-B; SFTB3; SFTP3; SMDP1; SP-B; OTTHUMP00000203253; Pulmonary surfactant-associated protein B; 18kD; surfactant; pulmonary-associated protein B; surfactant protein B

pulmonary surfactant-associated protein B isoform 1; Pulmonary surfactant-associated protein B; pulmonary surfactant-associated protein B; pulmonary surfactant-associated proteolipid SPL(Phe); surfactant associated protein B; Pulmonary surfactant-associated proteolipid SPL(Phe)

SFTPB

Sftpb; Sftpb; SF-B; SP-B; Sftp3; Sftp-3; AI562151; Sftp3; SP-B

PSPB_MOUSE

Mouse

377

This assay has high sensitivity and excellent specificity for detection of Mouse SFTPB. No significant cross-reactivity or interference between Mouse SFTPB and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 0.45 ng/ml-30 ng/ml. Sensitivity: 0.11 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 3: Gaseous exchange

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-B bound in the initial step. The color development is stopped and the intensity of the color is measured.

22296601

NP_680088.1

NM_147779.2

P50405

41,728 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

Function: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800