product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Pepsin, PG ELISA Kit
catalog :
MBS704203
quantity :
48-Strip-Wells
price :
565 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS704203
products type :
ELISA Kit
products full name :
Human Pepsin, PG ELISA Kit
products short name :
Pepsin, PG
products name syn :
Human Pepsin; PG ELISA Kit; Pepsin; PG
products gene name :
PG
reactivity :
Human
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, urine, tissue homogenates. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.81 pg/ml
other info2 :
Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Introduction: Pepsin is an enzyme that is released by the chief cells in the stomach and that degrades food proteins into peptides. Pepsin was discovered in 1836 by Theodor Schwann. It was the first animal enzyme to be discovered, and, in 1929, it became one of the first enzymes to be crystallized, by John H. Northrop. Pepsin is one of three principal protein-degrading, or proteolytic, enzymes in the digestive system, the other two being chymotrypsin and trypsin. Pepsin functions best in acidic environments and is often found in an acidic environment, particularly those with a pH of 1.5 to 2. Pepsin denatures if the pH is more than 5.0. Pepsin is said to have an optimum temperature between 37 degree C and 42 degree C in humans. Pepsin is potently inhibited by the peptide inhibitor pepstatin. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PG and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
size1 :
48-Strip-Wells
price1 :
565 USD
size2 :
96-Strip-Wells
price2 :
810
size3 :
5x96-Strip-Wells
price3 :
2750
size4 :
10x96-Strip-Wells
price4 :
5150
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!