ELISA/assay

Human cutaneous lymphocyte-associated antigen, CLA ELISA Kit

MBS704198

96 Strip Wells

655 USD

ELISA Kit

Human cutaneous lymphocyte-associated antigen, CLA ELISA Kit

chemokine (C-C motif) ligand 27

Human cutaneous lymphocyte-associated antigen; CLA ELISA Kit; ALP; CTACK; CTAK; ESKINE; ILC; PESKY; SCYA27; CC chemokine ILC; IL-11 Ralpha-locus chemokine; cutaneous T-cell attracting chemokine; skinkine; small inducible cytokine A27; small inducible cytokine subfa; Human cutaneous lymphocyte-associated antigen; CLA

C-C motif chemokine 27; C-C motif chemokine 27; C-C motif chemokine 27; skinkine; CC chemokine ILC; IL-11 Ralpha-locus chemokine; small-inducible cytokine A27; IL-11 R-alpha-locus chemokine; cutaneous T-cell attracting chemokine; cutaneous T-cell-attracting chemokine; small inducible cytokine subfamily A (Cys-Cys), member 27; chemokine (C-C motif) ligand 27; CC chemokine ILC; Cutaneous T-cell-attracting chemokine; CTACK; ESkine; IL-11 R-alpha-locus chemokine; Skinkine; Small-inducible cytokine A27

CCL27

CCL27; CCL27; ALP; ILC; CTAK; CTACK; PESKY; ESKINE; SCYA27; ILC; SCYA27; CTACK

CCL27_HUMAN

Human

112

This assay has high sensitivity and excellent specificity for detection of Human CCL27. No significant cross-reactivity or interference between Human CCL27 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Range: 0.313ng/ml-20 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.043ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Cytokine

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CLA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLA bound in the initial step. The color development is stopped and the intensity of the color is measured.

5730035

NP_006655.1

NM_006664.2

Q9Y4X3

12,618 Da

Chemokine Receptors Bind Chemokines Pathway 106359!!Chemokine Signaling Pathway 99051!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!G Alpha (i) Signalling Events Pathway 119550!!GPCR Downstream Signaling Pathway 119548!!GPCR Ligand Binding Pathway 161020!!Peptide Ligand-binding Receptors Pathway 106358

This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene is chemotactic for skin-associated memory T lymphocytes. This cytokine may also play a role in mediating homing of lymphocytes to cutaneous sites. It specifically binds to chemokine receptor 10 (CCR10). Studies of a similar murine protein indicate that these protein-receptor interactions have a pivotal role in T cell-mediated skin inflammation. [provided by RefSeq, Jul 2008]

Function: Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.

96 Strip Wells

655 USD