product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Insulin, INS ELISA Kit
catalog :
MBS704195
quantity :
24-Strip-Wells (LIMIT 1)
price :
240 USD
more info or order :
image
image 1 :
product information
catalog number :
MBS704195
products type :
ELISA Kit
products full name :
Human Insulin, INS ELISA Kit
products short name :
[insulin]
products name syn :
[Human Insulin; INS ELISA Kit; ILPR; IRDN; OTTHUMP00000011162; OTTHUMP00000196038; proinsulin; Human Insulin; INS]
other names :
[insulin preproprotein; Insulin; insulin; proinsulin; preproinsulin; insulin-dependent diabetes mellitus 2; insulin]
products gene name :
[INS]
other gene names :
[INS; INS; ILPR; IRDN; IDDM1; IDDM2; MODY10]
uniprot entry name :
INS_HUMAN
reactivity :
Human
sequence length :
110
specificity :
This assay has high sensitivity and excellent specificity for detection of human INS. No significant cross-reactivity or interference between human INS and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 8 muIU/ml -140 muIU/ml. Sensitivity: The minimum detectable dose of human INS is typically less than 5 muIU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human INS concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for INS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for INS. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INS bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
4557671
ncbi acc num :
NP_000198.1
ncbi gb acc num :
NM_000207.2
uniprot acc num :
P01308
ncbi mol weight :
11,981 Da
ncbi pathways :
AGE/RAGE Pathway (698754); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); ATF-2 Transcription Factor Network Pathway (138006); Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Amyloids Pathway (366238); Arf6 Trafficking Events Pathway (137954); Cardiac Progenitor Differentiation Pathway (712094)
ncbi summary :
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
uniprot summary :
Function: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
size1 :
24-Strip-Wells (LIMIT 1)
price1 :
240 USD
size2 :
48-Strip-Wells
price2 :
350
size3 :
96-Strip-Wells
price3 :
495
size4 :
5x96-Strip-Wells
price4 :
2105
size5 :
10x96-Strip-Wells
price5 :
3975
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!
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