Human membrane cofactor protein, MCP ELISA Kit | MyBioSource MBS704177 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human membrane cofactor protein, MCP ELISA Kit

catalog :

MBS704177

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704177

products type :

ELISA Kit

products full name :

Human membrane cofactor protein, MCP ELISA Kit

products short name :

CD46 molecule, complement regulatory protein

products name syn :

Human membrane cofactor protein; MCP ELISA Kit; AHUS2; MCP; MGC26544; MIC10; TLX; TRA2.10; CD46 antigen; complement regulatory protein; antigen identified by monoclonal antibody TRA-2-10; complement membrane cofactor protein; measles virus receptor; ; CD46 molecule; complement regulatory protein

other names :

membrane cofactor protein isoform 1; Membrane cofactor protein; membrane cofactor protein; measles virus receptor; complement membrane cofactor protein; trophoblast leucocyte common antigen; trophoblast leukocyte common antigen; CD46 antigen, complement regulatory protein; trophoblast-lymphocyte cross-reactive antigen; antigen identified by monoclonal antibody TRA-2-10; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); CD46 molecule, complement regulatory protein; TLX; Trophoblast leukocyte common antigen; CD_antigen: CD46

products gene name :

CD46

other gene names :

CD46; CD46; MCP; TLX; AHUS2; MIC10; TRA2.10; MCP; MIC10

uniprot entry name :

MCP_HUMAN

reactivity :

Human

sequence length :

392

specificity :

This assay has high sensitivity and excellent specificity for detection of human MCP. No significant cross-reactivity or interference between human MCP and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: 0.78 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

27502402

ncbi acc num :

NP_002380.3

ncbi gb acc num :

NM_002389.4

uniprot acc num :

P15529

ncbi mol weight :

43,747 Da

ncbi pathways :

Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Immune System Pathway (106386); Innate Immune System Pathway (106387); Measles Pathway (213306); Measles Pathway (213277); Regulation Of Complement Cascade Pathway (576254)

ncbi summary :

The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]

uniprot summary :

Function: Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.2 PublicationsManual assertion based on experiment in:Ref.29

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

615

size3 :

5x96-Strip-Wells

price3 :

2340

size4 :

10x96-Strip-Wells

price4 :

4370

more info or order :

MyBioSource product webpage