catalog number :
MBS704175
products type :
ELISA Kit
products full name :
Sheep Interferon gamma, IFN-gamma ELISA Kit
products short name :
interferon, gamma
products name syn :
Sheep Interferon gamma; IFN-gamma ELISA Kit; IFG; IFI; ; interferon; gamma
other names :
interferon gamma; Interferon gamma; interferon gamma; gamma-interferon
products gene name :
IFNG
other gene names :
IFNG; IFNG; IFN-gamma; IFN-gamma
uniprot entry name :
IFNG_SHEEP
specificity :
This assay has high sensitivity and excellent specificity for detection of sheep IFN-gamma. No significant cross-reactivity or interference between sheep IFN-gamma and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 5 pg/ml -200 pg/ml. Sensitivity: The minimum detectable dose of sheep IFN-gamma is typically less than 2.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest sheep IFN-gamma concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFN-gamma has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for IFN-gamma. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-gamma bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001009803.1
ncbi gb acc num :
NM_001009803.1
ncbi mol weight :
19,370 Da
ncbi pathways :
ATF-2 Transcription Factor Network Pathway (138006); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993)
uniprot summary :
Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
size4 :
10x96-Strip-Wells