ELISA/assay

Human Elastin, ELN ELISA Kit

MBS704171

48-Strip-Wells

565 USD

ELISA Kit

Human Elastin, ELN ELISA Kit

elastin

Human Elastin; ELN ELISA Kit; FLJ38671; FLJ43523; SVAS; WBS; WS; OTTHUMP00000211864; tropoelastin; elastin

elastin isoform a; Elastin; elastin; tropoelastin; elastin; Tropoelastin

ELN

ELN; ELN; WS; WBS; SVAS

ELN_HUMAN

Human

724

This assay has high sensitivity and excellent specificity for detection of human ELN. No significant cross-reactivity or interference between human ELN and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, urine, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: 0.78 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ELN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ELN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ELN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ELN bound in the initial step. The color development is stopped and the intensity of the color is measured.

126352440

NP_000492.2

NM_000501.3

P15502

68,469 Da

Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868)

This gene encodes a protein that is one of the two components of elastic fibers. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function: Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150