catalog number :
MBS704163
products type :
ELISA Kit
products full name :
Rat Epinephrine/Adrenaline, EPI ELISA Kit
products short name :
Epinephrine/Adrenaline, EPI
products name syn :
Rat Epinephrine/Adrenaline; EPI ELISA Kit; Epinephrine/Adrenaline; EPI
other names :
EPI protein; EPI protein; EPI protein
uniprot entry name :
B9DR51_LEPMC
specificity :
This assay has high sensitivity and excellent specificity for detection of rat EPI. No significant cross-reactivity or interference between rat EPI and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Detection Range: 1 pg/ml -200 pg/ml. Sensitivity: The minimum detectable dose of rat EPI is typically less than 0.5 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest rat EPI concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Competitive
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for EPI and Horseradish Peroxidase (HRP) conjugated EPI. The competitive inhibition reaction is launched between with HRP labeled EPI and unlabeled EPI with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of EPI in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
CAQ03441.1
ncbi mol weight :
41,398 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells