catalog number :
MBS704159
products type :
ELISA Kit
products full name :
Human Lithostathine-1-alpha (REG1A) ELISA Kit
products short name :
Lithostathine-1-alpha (REG1A)
products name syn :
Human Lithostathine-1-alpha (REG1A)
other names :
REG1A protein; REG1A protein; lithostathine-1-alpha; protein-X; REG-1-alpha; OTTHUMP00000160485; lithostathine 1 alpha; pancreatic thread protein; regenerating protein I alpha; islet cells regeneration factor; pancreatic stone protein, secretory; islet of langerhans regenerating protein; regenerating islet-derived protein 1-alpha; regenerating islet-derived 1 alpha
products gene name :
REG1A
other gene names :
REG1A; REG1A; P19; PSP; PTP; REG; ICRF; PSPS; PSPS1; MGC12447
uniprot entry name :
Q2TBE1_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human REG1A. No significant cross-reactivity or interference between human REG1A and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: The minimum detectable dose of human REG1A is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products categories :
Human ELISA Kit
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for REG1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any REG1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
9,676 Da
ncbi pathways :
Insulin Signaling Pathway (198845); Type II Interferon Signaling (IFNG) Pathway (198918)
ncbi summary :
This gene is a type I subclass member of the Reg gene family. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins. This gene encodes a protein that is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. Reg family members REG1B, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication. [provided by RefSeq]
uniprot summary :
REG1A: Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2p12. Molecular Function: growth factor activity; carbohydrate binding. Biological Process: positive regulation of cell proliferation
size4 :
10x96-Strip-Wells