catalog number :
MBS704157
products type :
ELISA Kit
products full name :
Mouse Arachidonic Acid, AA ELISA Kit
products short name :
Arachidonic Acid (AA)
products name syn :
Mouse Arachidonic Acid (AA) ELISA Kit; Mouse Arachidonic Acid (AA)
other names :
arachidonic acid epoxygenase, partial; Cytochrome P450 2C23; Arachidonic acid epoxygenase; CYPIIC23
other gene names :
Cyp2c23; Cyp2c-23
uniprot entry name :
CP2CN_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse AA. No significant cross-reactivity or interference between mouse AA and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 15.625 ng/ml-1000 ng/ml. Sensitivity: The minimum detectable dose of mouse AA is typically less than 13.797 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest mouse AA concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AA. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated AA. The competitive inhibition reaction is launched between with HRP-conjugated AA and AA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of AA in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAB29022.1
ncbi mol weight :
56,433 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Predominant enzyme isoform responsible for arachidonic acid epoxidation in the rat kidney. Catalyzes the NADPH-dependent metabolism of arachidonic acid to a mixture of 5,6-, 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acids (EETS) as the only oxygenation products. Low rate of lauric acid hydroxylation at the (omega-1)-position. Catalytic activity: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. Cofactor: Heme group
size3 :
10x96-Strip-Wells