catalog number :
MBS704153
products type :
ELISA Kit
products full name :
Rat 1, 25-dihydroxyvitamin D3, 1, 25 DHVD3 ELISA Kit
products short name :
1, 25-dihydroxyvitamin D3
products name syn :
Rat 1; 25-dihydroxyvitamin D3 (1; 25 DHVD3) ELISA Kit; Rat 1; 25-dihydroxyvitamin D3 (1; 25 DHVD3)
products gene name :
1, 25 DHVD3
specificity :
This assay has high sensitivity and excellent specificity for detection of rat 1,25 DHVD3. No significant cross-reactivity or interference between rat 1,25 DHVD3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum. Assay Type: Competitive. Detection Range: 1000 fmol/L - 5000 fmol/L. Sensitivity: The minimum detectable dose of rat 1,25 DHVD3 is typically less than 250 fmol/L. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest rat 1,25 DHVD3 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated 1,25 DHVD3 Hapten. The competitive inhibition reaction is launched between with HRP-conjugated 1,25 DHVD3 Hapten and 1,25 DHVD3 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of 1,25 DHVD3 in the sample. The color development is stopped and the intensity of the color is measured.
size3 :
10x96-Strip-Wells