ELISA/assay

Human von Willebrand Factor, vWF ELISA Kit

MBS704140

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human von Willebrand Factor, vWF ELISA Kit

[von Willebrand factor]

[Human von Willebrand Factor; vWF ELISA Kit; F8VWF; VWD; coagulation factor VIII VWF; von Willebrand factor]

[von Willebrand factor preproprotein; von Willebrand factor; von Willebrand factor; coagulation factor VIII VWF; von Willebrand factor; von Willebrand antigen II]

[VWF]

[VWF; VWF; VWD; F8VWF; F8VWF; vWF]

VWF_HUMAN

Human

2813

This assay has high sensitivity and excellent specificity for detection of mouse VLDL. No significant cross-reactivity or interference between mouse VLDL and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.78 ng/ml-50 ng/ml. Sensitivity: 0.195 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VLDL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VLDL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VLDL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VLDL bound in the initial step. The color development is stopped and the intensity of the color is measured.

89191868

NP_000543.2

NM_000552.3

P04275

309,265 Da

Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057)

The glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]

Function: Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

450

96-Strip-Wells

640

5x96-Strip-Wells

2615

10x96-Strip-Wells

4940