Human Glutamate--cysteine ligase Catalytic subunit, GCLC ELISA Kit | MyBioSource MBS704124 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Glutamate--cysteine ligase Catalytic subunit, GCLC ELISA Kit

catalog :

MBS704124

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704124

products type :

ELISA Kit

products full name :

Human Glutamate--cysteine ligase Catalytic subunit, GCLC ELISA Kit

products short name :

glutamate-cysteine ligase, catalytic subunit

products name syn :

Human Glutamate--cysteine ligase catalytic subunit (GCLC) ELISA kit; GCL; GCS; GLCL; GLCLC; gamma-glutamylcysteine synthetase; glutamate-cysteine ligase; catalytic subunit

other names :

glutamate--cysteine ligase catalytic subunit isoform b; Glutamate--cysteine ligase catalytic subunit; glutamate--cysteine ligase catalytic subunit; gamma-ECS; GCS heavy chain; gamma-glutamylcysteine synthetase; glutamate-cysteine ligase, catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase

products gene name :

GCLC

other gene names :

GCLC; GCLC; GCL; GCS; GLCL; GLCLC; GLCL; GLCLC

uniprot entry name :

GSH1_HUMAN

reactivity :

Human

sequence length :

599

specificity :

This assay has high sensitivity and excellent specificity for detection of human GCLC. No significant cross-reactivity or interference between human GCLC and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human GCLC is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GCLC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GCLC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GCLC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GCLC bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

308199423

ncbi acc num :

NP_001184044.1

ncbi gb acc num :

NM_001197115.1

uniprot acc num :

P48506

ncbi mol weight :

72,766 Da

ncbi pathways :

Biological Oxidations Pathway (105698); Glutathione Biosynthesis, Glutamate = Glutathione Pathway (413393); Glutathione Biosynthesis, Glutamate = Glutathione Pathway (468307); Glutathione Conjugation Pathway (105727); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343); Glutathione Synthesis And Recycling Pathway (105728); Keap1-Nrf2 Pathway (198777); Metabolic Pathways (132956)

ncbi summary :

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]

uniprot summary :

GCLC: Defects in GCLC are the cause of hemolytic anemia (HAGGSD). Belongs to the glutamate--cysteine ligase type 3 family. Protein type: Other Amino Acids Metabolism - glutathione; EC 6.3.2.2; Ligase. Chromosomal Location of Human Ortholog: 6p12. Cellular Component: glutamate-cysteine ligase complex; cytosol. Molecular Function: glutamate binding; protein heterodimerization activity; glutamate-cysteine ligase activity; magnesium ion binding; ADP binding; coenzyme binding; ATP binding. Biological Process: glutamate metabolic process; response to arsenic; response to hormone stimulus; L-ascorbic acid metabolic process; response to nitrosative stress; cysteine metabolic process; apoptotic mitochondrial changes; sulfur amino acid metabolic process; cell redox homeostasis; response to heat; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; xenobiotic metabolic process; glutathione biosynthetic process; negative regulation of protein ubiquitination; response to oxidative stress; negative regulation of neuron apoptosis; regulation of blood vessel size; negative regulation of transcription, DNA-dependent; regulation of mitochondrial depolarization; negative regulation of apoptosis. Disease: Gamma-glutamylcysteine Synthetase Deficiency, Hemolytic Anemia Due To; Myocardial Infarction, Susceptibility To

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage